摘要
目的探讨缺氧诱导因子1α(HIF1α)在胃癌组织中的表达及其临床意义。方法采用免疫组化SP法,检测74例经手术切除的人体胃癌组织(术前均未行放疗及化疗)中HIF1α的表达。结果HIF1α主要表达于胃癌细胞浆及胞核,肿瘤间质细胞也可见少量表达,肿瘤坏死区周围及肿瘤浸润边缘部的癌细胞HIF1α表达明显增多。HIF1α的表达强度与细胞分化程度(P<0.05)、胃癌淋巴结转移(P<0.05)及预后(P<0.01)有关,而与病人年龄、性别及原发肿瘤大小均无关。其阳性强度随癌细胞分化程度的降低、淋巴结转移、术后生存期短而增高。结论HIF1α可作为评估胃癌淋巴结转移和预后的重要指标。
Objective To investigate the significance of hypoxia-inducible factor-1α(HIF-1α)expression in gastric carcinoma.Methods The expression of HIF-1α was detected immunohistochemically by SP in 74 patients with gastric carcinoma.Results HIF-1α was mainly expressed in the cytoplasm and nucleus of tumor cells,but little in the cytoplasm of mesenchymal cells.HIF-1α was evidently expressed in tumor cells at the periphery of necrosis and the margin of the tumor.The expressive intensity was significantly higher in gastric low differentiated carcinoma than well-defferentiated carcinoma(P<0.05).The expressive intensity was related to the malignancy of the cancer cell(P<0.05),lymph node metastasis(P<0.05),and prognosis(P<0.01),but not to the age or sex of the patients,or the size of the primary tumors.The expressive intensity increased with the decrease of the differentiation of cancer cells,lymph node metastasis and the decrease of post-operative survival time.Conclusion The overexpression of HIF-1α may serve as an important parameter in evaluating lymph node metastasis and prognosis of gastric carcinoma.
出处
《福建医药杂志》
CAS
2005年第3期6-8,共3页
Fujian Medical Journal
