摘要
目的评价人工血管内支架与腹主动脉的生物相容性。方法用膨体聚四氟乙烯膜压于镍钛合金支架制成人工血管内支架,将其植入实验猪肾下腹主动脉。2、4、12周取材,切片行苏木素伊红(HE)染色、胶原纤维和弹力纤维染色、血管平滑肌细胞α肌动蛋白免疫组织化学检查以及细胞原位凋亡检测,并行图像分析,对照实验组与正常腹主动脉内膜厚度、胶原纤维和弹力纤维相对含量、血管平滑肌细胞密度和凋亡比例。结果实验组2周时人工血管内支架表面有内皮细胞覆盖,与正常组相比,其各时间点内膜增生显著(P<0.01),弹力纤维和胶原纤维的形态和分布基本正常(P>0.05),2、4周时中膜内血管平滑肌细胞密度和凋亡比例显著高于正常组(P<0.01)。结论人工血管内支架与腹主动脉有着良好的生物相容性,但内膜增生较明显。
Objective To evaluate the biocompatibility between the domestic stent-graft and abdominal aorta.Methods We made stent-graft with felting of expanding-polytetra- fluroethylene (ePTFE) graft and nitinol stent.They were implanted in the subrenal abdominal aorta.The specimens were taken out 2,4,12 weeks afterwards and underwent HE/special pigmentation of collagen and elastin,immunohistochemistry of α actin from vascular smooth muscle cells (VSMC),in-situ apoptosis detection and scan of electromicroscopy.The comparison between them and normal abdominal aorta in intimal thickness,relative content of collagen and elastin,density and apoptosis ratio of VSMC in medium was made through the image analysis.Results The stent-graft was covered with intima as early as 2 weeks but suffered more severe intimal hyperplasia all the time (P< 0.01).The morphology and distribution of collagen and elastin were generally normal in the experimental group (P> 0.05).However,its density and apoptosis ratio of VSMC in medium were significantly higher than in the normal ones (P< 0.01) in 2,4 weeks.Conclusion The stent-graft shared fine biocompatibility with the abdominal aorta except for the significant intimal hyperplasia.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2005年第6期657-658,共2页
Chinese Journal of Experimental Surgery
基金
上海市科委基金资助项目(004419031)
