摘要
目的探讨高复发膀胱移行细胞癌基因组非平衡性的变化,寻找与膀胱癌复发相关的染色体位点或区段的畸变。方法选择20例膀胱移行细胞癌患者的石蜡包埋组织标本,经随访分为高复发组11例,无复发组9例。应用比较基因组杂交(CGH)对每一个标本进行检测,得出其染色体畸变的位点或区段,应用χ2确切概率法统计高复发组与无复发组畸变的差异。结果增益频率较高的染色体区段或位点位于1p、1q、3p、5p、5q、8q、17q;缺失频率较高的染色体区段或位点位于4q、8p、9q、11q、11p、17p。高复发组与无复发组相比较,9q、11p缺失的差异有统计学意义(P<0.05);而9q、11p同时缺失的差异无统计学意义(P>0.05)。结论染色体9q、11p上某些区段或位点的缺失与膀胱癌复发有关,此2个染色体臂上可能存在膀胱移行细胞癌的“复发相关基因”,其上的基因缺失或失活可能导致膀胱移行细胞癌的复发。
Objective To investigate the change of genomic imbalance on high recurrent transitional cell carcinoma of urinary bladder,and find the correlation between bladder cancer recurrence and the aberrations of chromosomal sections or loci.Methods Twenty cases of formalin fixed,paraffin-embedded specimens of bladder cancer were selected,which were divided into high recurrence group and non recurrence group after follow-up.The method of comparative genomic hybridization (CGH) was used to detect the every specimen to get the aberration of chromosomes.The difference of the aberration by chi-square exact probabilities test was measured.Results[WT5”BZ] Among all the bladder cancer tissues,the most frequent chromosomal gainslocated in 1p,1q,3p,5p,5q,8q and 17q;However,the most frequent chromosomal losses located in 4q,8p,9q,11q,11p and 17p.Between the non recurrence group and high recurrence group,there was significant difference in the 9q and 11p loss (P< 0.05),but there was no significant difference in 9q plus 11p loss between them (P> 0.05).Conclusion The chromosomal sections or loci losses on 9q and 11p had relationship with bladder cancer recurrence.There were likely to harbor 'recurrence-related genes' of bladder cancer on the 2 arms.The losses or inactivation of the genes on the arms might result in the recurrence of transitional cell carcinoma of urinary bladder.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2005年第7期871-872,共2页
Chinese Journal of Experimental Surgery
基金
上海市自然科学基金资助项目(03ZR14018)
