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韭菜白绢病菌的分离及其特性鉴定 被引量:12

Isolation and characterization of Sclerotium rolfsii causing leek blight
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摘要 主要研究了温度、pH值、光照、湿度、碳氮源以及药剂对韭菜白绢病菌(SclerotiumrolfsiiSacc )生长的影响以及该菌对不同药剂的敏感性。该菌菌丝生长的温度范围是13~40℃,最适31℃;pH值范围是2~9,最适pH为6;红光最利于菌丝生长;对山梨醇、蔗糖利用较好,不能利用乳糖;能利用多种氮源,以蛋白胨利用最好。菌核萌发的温度范围为16~40℃,最适34℃;pH值范围为2~10,最适pH值是3~4;湿度为100%+水滴的条件下才能萌发;绿光最利于菌核萌发,另外,光照利于菌核产生,白光下产生的菌核数最多。11种参试药剂中抑菌效果较好的有:丙环唑、咪鲜胺、腈菌唑、三唑酮和苯醚甲环唑。 Effects of temperature, pH, light,moisture , nutrient and fungicides on growth of Sclerotium rolfsii Sacc. were investigated. The range of temperature for the mycelium growth was 13-40 ℃, the optimum at 31 ℃. The range of pH was 2-9, the optimum at pH 6. Red light was the best light to it's growth. The best carbon source was sorbierte and sucrose. The fungus could not survive on lactose. Among the 9 nitrogen sources tested, peptone gave the best growth. The range of temperature for sclerotia germination was 16-40 ℃,the optimum at 34 ℃.The range of pH was 2-10, the optimum at pH 3-4.Under RH 100 %+water, the sclerotia could germinate well. Green light was the best light to it's germination. In addition, light was good for sclerotia production. White light was the best light to the sclerotia production.Among the 11 fungicides tested, these fungicides, such as propiconazole, myclobutanil, triadimefon, difenoconazole and prochloraz, gave a strong inhibition to the growth of the fungus.
出处 《西南农业学报》 CSCD 2005年第3期302-305,共4页 Southwest China Journal of Agricultural Sciences
关键词 韭菜 白绢病菌 生物学特性 leek Sclerotium rolfsii biological character
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