摘要
由Ca2+/H+反向转运体蛋白的特异多肽制备的抗体,通过免疫印迹显示水稻根质膜上存在分子量为44.5kD的蛋白。同位素闪烁记数法检测水稻根质膜上45Ca2+转运活性显示存在依赖于跨膜质子梯度的Ca2+/H+反向转运体,Mn2+可部分地抑制其转运活性。动力学分析表明,OsCAX2的Vmax=34.72nmol/(min·mg),Km=3.83μmol/L,其最适pH为7.2。
The peptide-sepcific antipobies of the Ca^(2+)/H^+ antiporter clearly reacted with a 44.5 kD protein from rice root plasma membrane by immunoblotting. Ca^(2+) transport activities of rice root plasma membrane by the filtration method using ^(45)Ca^(2+), indicated that there was a Ca^(2+)/H^+ antiporter(OsCAX2) depended on transmembrane proton gradient in the rice root plasma membrane, and manganese ion could restrain Ca^(2+) transport activities of OsCAX2 partially. Kinetics analysis showed that the V_(max) of OsCAX2 was 34.72 nmol/(min·mg), the K_m was 3.83 μmol/L and the optimum pH value was 72.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2005年第4期308-314,共7页
Chinese Journal of Rice Science
基金
国家基础研究发展规划项目(G1999011700)
台州学院校立项目(04ND38)。
