摘要
超藤葡萄是藤稔葡萄的一个芽变品种,由于2者亲缘关系十分接近,应用形态学手段以及RAPD分子标记不易区分。针对葡萄叶片富含多糖和多酚类等杂质的特点,从基因组DNA纯化入手,对AFLP流程进行了优化改进,建立了葡萄AFLP体系,并成功地对此2个品种进行了区分。采用引物组合M-CAT+E-ACG进行AFLP扩增,超藤葡萄出现167bp特异带。
Chaoteng grape, as a somatic mutant of Fujiminori, is quite difficult to be distinguished from its parental line by morphological or even RAPD methods. Here we provided an improved AFLP protocol suitable for grape whose leaves are highly rich in impurities including polysaccharides and polyphenols. And the two grape cultivars were successfully discriminated when AFLP primers M-CAT and E-ACG were applied,where Chaoteng produced a specific AFLP product of 167 bp in length.
出处
《果树学报》
CAS
CSCD
北大核心
2005年第4期422-425,共4页
Journal of Fruit Science
关键词
葡萄
AFLP
品种鉴别
Grape (Vitis vinifera L.)
AFLP
Cultivar distinguishing
