摘要
目的筛选紫草抗人乳头瘤病毒(HPV)的有效部位。方法用系统溶媒(石油醚、正丁醇、乙醇、蒸馏水)对紫草依次进行提取,应用荧光定量PCR技术检测在体外的抗HPV-DNA的效果。结果仅紫草水提物显示在体外抑制HPV-DNA活性,最低有效浓度为0.08g/mL。结论紫草具有体外抑制HPV-DNA作用,其水提物中存在抗HPV-DNA活性成分。
Objective To select the herba anti- human papillomavirus (HPV) active fraction from herba Arnebia. Methods The fractions from herba Arnebia. were separated with systematic solvents including petroleum arieal part of benzin, n- butanol , ethanol and distilled water, and their effects on HPV- DNA were evaluated by fluorescence quantitative polymerase chain reaction (FQ- PCR) technique. Results Only the water- extract of.this drug showed in- vitro inhibitory effect on HPV- DNA and its minimum effective concentration is 0.08g/mL. Conclusion Herba Arnebia. has in- vitro inhibitory effect on HPV- DNA and the active components exists in the water- extract.
出处
《中药新药与临床药理》
CAS
CSCD
2005年第4期259-260,共2页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广东省医学科研基金资助项目(编号A2003260)。
关键词
紫草提取物
人乳头瘤病毒
荧光定量聚合酶链反应
Herba Arnebia
Human papillomavirus
Fluorescence quantitative polymerase chain reaction
