摘要
建立了马拉硫磷对映体的高效液相色谱正相体系拆分方法。分别应用了正己烷和石油醚两种流动相,在正己烷流动相体系中采用了乙醇、丙醇、异丙醇、丁醇和异丁醇有机改性剂,石油醚体系则以异丙醇为改性剂,考察了改性剂及其含量对拆分的影响。同时还考察了温度对分离的影响。实验结果显示,室温下正己烷流动相中含5%异丁醇可达最佳分离效果,分离因子为1.22。流动相中醇的含量减少使保留时间延长,分离效果增加。低温有利于对映体的分离。
The method of chiral separation for the two enantiomers of malathion was set up by high performance liquid chromatography(HPLC).Petroleum ether and n-hexane were used as mobile phase.Ethanol,propanol,2-propanol,butanol and 2-butanol were added as modifiers in n-hexane and 2-propanol was used in petroleum ether as modifier.The influence of modifiers and their content on the separation was investigated,the effect of temperature was also studied.The results showed that 2-propanol,butanol and 2-butanol were appropriate for the separation.When the concentration of modifier in mobile phase decreased the retention and resolution increased.Low temperature was good favorable for the resolution.
出处
《化学试剂》
CAS
CSCD
北大核心
2005年第7期385-386,439,共3页
Chemical Reagents
基金
国家自然科学基金资助项目(20377052)。
