摘要
本研究结果发现，黄芪对柯萨奇Ｂ_３病毒（ＣＶＢ_３）感染大鼠心肌细胞及正常对照心肌细胞的Ｃａ^（２＋）内流均呈显著抑制作用（ｐ＜０．０１，Ｐ＜０．０５））；若在病毒感染１ｈ后加黄芪，经４８ｈ培养，对正常对照及感染细胞的Ｃａ^（２＋）内流亦有抑制作用（Ｐ＜０．０５）；同时细胞中ＣＶＢ_３－ＲＮＡ含量显著少于病毒对照组（Ｐ＜０．００１）。提示黄芪具有钙拮抗作用，可减少病毒感染引起的心肌Ｃａ^（２＋）内流量，有可能减轻感染细胞的继发性Ｃａ^（２＋）损伤；且对细胞中病毒ＲＮＡ的复制具有抑制作用，显示了其在病毒性心肌炎临床治疗上的应用价值。
The effect of Astrapalus membranaceus(AM) on Ca2+ influx across the myocardial plasma membrane and coxsackie virus B3(CVB3)-RNA replication in cultured neonatal rat heart cells infected with CVB3 was investigated.It was found that the Ca2+ influx could be inhibited significantly(P<0.01) by AM after infection of heart cells for 48 h.In addition,when the cultured heart cells infected with CVB3 and treated with AM for 48 h,the Ca2+ influx of infected heart cells also could be inhibited by AM(P<0.05) and the amounts of CVB3-RNA in myocytes were significantly decreased than that in infected control group(P<0.001).These phenomena suggested that AM could exert the effects of decreasing the secondary Ca2+ damages,and improving the abnormal myocardial electric activity,and inhibiting replication of CVB3-RNA in myocardium.Thus, it is a rational choice to treat patients with AM in viral myocarditis.
出处
《中国中西医结合杂志》
CSCD
北大核心
1995年第8期483-485,共3页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金
关键词
心肌炎
病毒性心肌炎
黄芪
钙内流
CVB3
Coxsackie virus B_3
viral myocarditis
Ca￣(2+) influx
Astragalus membranaceus
