摘要
自人肺巨细胞癌系分离了多个单细胞克隆，经体外侵袭实验和裸鼠体内接种相结合进行了初步鉴定后，挑选其中３个克隆化细胞亚系（ＰＧＢＥ＿１、ＰＧＣＬ＿３和ＰＧＬＨ＿７）进行形态、染色体众数、体外生长和侵袭，以及棵鼠体内成瘤率和自发性转移率检测。结果显示ＰＧＢＥ＿１、ＰＧＣＬ＿３和ＰＧＬＨ＿７在裸鼠体内的成瘤率均为１００％，淋巴结转移率分别为９４％、９４％和５０％，肺转移率则分别为８８％、６７％和４４％。表明人肺巨细胞癌母系为一异质性的细胞群体。
ingle cell clones were isolated from metastatic human lung giant cell carcinoma(PG)using limited dilution method。After preliminary screening by in vitro invasion assay,tumorigenicity and spontaneous metastasis assay in nude mice,three cloned PG cell sublines(PGBE_1,PGCL_3 and PGLH_7)were selected for further observation on morphology,chromosome number,in vitrO growth and invasion assys,tu-morigenicity and spontaneous metastasis to lung and lymph nodes in nude mice,We found that there was some difference on spontaneous metastasis to lung and lymph nodes in nude mice and in vitro inva-sive abilities between PGBE_1 and PGLH_7.But there were no obvious differences in other biological fea-tures among the three sublines.The results show that the PG cell line consists of different subpopula-tions of cells with differrent metastatic properties. These sublines may be valuable for further study on the molecular mechanisrns of cancer metastasis。
出处
《中华病理学杂志》
CAS
CSCD
北大核心
1995年第3期136-138,共3页
Chinese Journal of Pathology
基金
国家"八.五"攻关课题
国家自然科学基金
