摘要
目的探讨热休克蛋白70(heat shockprotein70,HSP70)和Ki-67在鼻咽癌组织中的表达及与预后的关系。方法采用免疫组织化学二步法,观察50例鼻咽癌患者鼻咽部或颈淋巴结活体组织检查存档石蜡标本中HSP70和Ki-67蛋白的表达。结果50例鼻咽癌组织中HSP70和Ki-67阳性表达率分别为36.0%(18/50)和98.0%(49/50);HSP70在T分期中的阳性表达差异无统计学意义;HSP70(+)组生存率(10.9%)与HSP70(-)组(33.8%)相比,差异有统计学意义,χ2=4.10,P=0.043;Cox模型多因素分析显示,HSP70(+)表达是独立的预后不良因素之一,P=0.031。在临床研究指标(T分期、N分期、生存率等)中,Ki-67的高表达(++/+++)率与低表达(+/-)率比较均差异无统计学意义。结论HSP70表达可作为评价鼻咽癌患者预后的指标。
OBJECTIVE: To explore the expressions of heat shock protein 70 (HSP70) and Ki-67 in nasopharyngeal carcinoma and the relation with prognosis. METHODS: Immunohistochemical study for HSP70 and Ki-67 was performed on buffered formalin fixed, paraffin embedded sections of 50 cases of nasopharyngeal carcinoma. RESULTS: The positive rates of HSP70 and Ki-67 in 50 patients with nasopharyngeal carcinoma were 36.0%(18/50) and 98.0%(49/50) respectively. The difference of positive expression of HSP70 in the T stage was not significant. Patients with negative expression for HSP70 had much higher 5-year survival rates than those with positive expression, χ2=4.10, P=0.043. Multivariate analysis identified that the positive expression of HSP70 was one of poor prognostic factors, P=0.031. There was no significant correlation between the positive expression of Ki-67 and clinical findings, such as T stage, N stage and 5-year survival rates. CONCLUSION: The expression of HSP70 may be helpful to predict the prognosis of patients with nasopharyngeal carcinoma.
出处
《肿瘤防治杂志》
2005年第14期1076-1079,共4页
China Journal of Cancer Prevention and Treatment
