摘要
观察了２１名海员在海上航行平稳和颠簸明显时期红细胞乙酰胆硷酯酶活性的变化。结果表明颠簸所造成的前庭刺激可引起该酶活性降低（Ｐ＜０．０５）。动物实验发现，大鼠接受正负交变加速度旋转刺激后，红细胞和丘脑、延脑内乙酰胆硷酯酶活性均明显降低（Ｐ＜０．０１）。结果表明，人和动物机体受到异常前庭刺激时红细胞与中枢某些部位的乙酰胆硷酯酶活性发生的变化类似。
he effects of vestibular stimulation on the activity
of acetyl cholinesterase(ACHE)in red blood cells(RBC)and brain were observed and presented
as follows:.The RBC AChE activity of 21 seamen at rough sea was fouud decreased
significantly(67.8± 12.8 U/ml RBC)as compared with that at calm sea(74.3±8.2U/ml
RBC)(P<0.05).And this re- sult showed that the vestibular stimulation induced by pitch and roll
of the ship might be responsible for the reduction. Ⅱ.The effects of rotatory stimulation on
AChE activities in RBC and brain of rats. The experiments were done in 20 Wistar-strain
rats.The rotatory device was modelled on Crampton's report.The rats were placed at random in
the device in each experiment.Rotatory veIocity was acceler- ated by positive angular
acceleration with 16°/s. Once the animals were brought to the peak velocity of 120°/s,then
they were decelerated by negative angular acceleration to zero,And this acceleration and
deceleration aIternativ process continued for 1 h.All the experimental animals were decapitated
soon after the cessation of rotation. The brain tissue was separpated and blood specimens
were collected.The activity of AChE was measured by the improved method of Ellman.
Results:(1)The RBC AChE activity of the experiment group (n=10)was decreased significantly to
15.9±1.7 U/ml RBC(P<0.01).(2)The AChE activities in thalamus and medulla oblongata of the
experiment group(n=10)were decreased significantly to 168.9±11. 9 U/g and 150. 0±12.8 U/g
brain wet weight respectively as compared with that of 185.3±7.3U/g and 163.8±8.1U/g brain
wet weight in the control group(n=10)(P<0.01)。These results indicate that when the body
receives abnormal vestibular,stimulation,similar ACHE activity changes will occur in the RBC
and in some structures of the central nervous system.
关键词
前庭刺激
乙酰胆硷酯酶
红细胞
脑
航海医学
Vestibular stimulation Acetyl
cholinesterase Red blood cell Brain
