摘要
目的:探讨五灵胶囊中柴胡皂苷d在小鼠体内药代动力学特征。方法:小鼠ig五灵胶囊,在不同时间点采集血浆,加硫酸胺与乙腈甲醇(4∶1)混合液萃取制备样品液,经RPHPLC测定血浆中柴胡皂苷d的浓度,SIP7软件拟合药动学参数。结果:小鼠ig给药五灵胶囊(5.5g/kg),五灵胶囊中柴胡皂苷d的药代动力学模型为一级吸收单室模型,其药代动力学参数在第1吸收峰Ka=2.341/h,Ke=0.081/h,Tmax=1.45h,Cmax=83.81ug/ml,T1/2Ke=7.76h;在第2吸收峰Ka=1.321/h,Ke=0.081/h,Tmax=6.48h,T1/2Ke=7.76h。结论:五灵胶囊中柴胡皂苷d在小鼠体内生物药剂学特征是吸收慢,清除也慢。
AIM To investigate the pharmacokinetic characters of saikosaponin d in Wuling Capsules in mouse. METHODS Wuling Capsules were given by irrigated (ig) administered to mouse, the blood plasma was collected at different time and the sample solution was prepared by extract adding amine sulfate and the mix fluid of acetonitrile-methyl alcohol (4∶ 1), the content of saikosaponin d was detected by RP-HPLC. The pharmacokinetic parameter was fitted by SIP7 software. RESULTS After ig administered the Wuling Capsules (5.5 g/kg) to mouse, the blood plasma concentration-time course fitted well to one compartment model with the 1st order absorption. The pharmacokinetic parameters of the first absoption in mouse was as following: Ka=2.34_ 1/h ,Ke=0.08_ 1/h ,T_ max =1.45_h,C_ max =83.81_ μg/ml ,T_ 1/2Ke =7.9 h; and that of the second absorption was as following Ka=1.32_ 1/h ,Ke=0.08_ 1/h ,T_ max =6.48h,C_ Max =119.63_ μg/ml , T_ 1/2 Ke =7.76 h. CONCLUSION The boipharmaceutics characters of saikosaponin d of Wuling Capsules were absorption slow and elimination was also slow.
出处
《中成药》
CAS
CSCD
北大核心
2005年第7期809-811,共3页
Chinese Traditional Patent Medicine
