摘要
应用聚合酶链反应（ＰＣＲ）技术检测临床标本（支气管肺泡灌洗液、咽拭子）中的肺炎支原体（ＭＰ）。在１４０例非细菌性肺炎患者的标本中，ＰＣＲ试验阳性３０例。其中间接血凝试验（检测ＭＰ抗体）阴性而ＰＣＲ试验阳性者２１例。ＰＣＲ阳性标本经ＭＰ５－４寡核苷酸探针检测验证，均为阳性结果。
he polymerase chain reaction (PCR) techniquewas used to detect Mycoplasma pneumoniae in clinicalsamples (bronchoalveolar lavage fluids and throatswabsl. A specific DNA sequence for M. pneumoniaewas selected from a genomic library. The amplificationtarget region was partial DNA sequece of the 500- bpfragment. The oligonucleotide sequence of two primerstMPS-1 and MPS-2) were complementary with theoligonucleotide sequence in two ends of the amplificationtarget region. PCR with purified DNA fragment astemplates yielded an expected 144-bp fragment from M.pneumoniae but not from any of the other Mycoplasmespp. assayed. With this method, the 144-bp productspecific for M. pneumoniae could be obtained from aminimum of 10 pg of M. pneumoniae DNA. Subse-quently this PCR technique was used for the detection ofM. pneumoniae in brochoalveolar lavage fluids or inthroat swab samples. Thirty of 140 samples from thepatients with non-bacterial pneumonia gave positive re-sults in the test. Twenty-one indirect hemoagglutinationtest-negative clinical samples from the same patients of140 cases gave positive results in the same PCR test.When the amplified preducts were hybridized with acomplementary probe (MP5- 4 oligonucleotide probe),thirty PCR- positive samples all gave positive hybridiza-tion signals. It suggests that PCR method can be usedfor the direct detection of M. pneumoniae in clinicalsamples.
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
1995年第1期41-43,共3页
Chinese Journal of Tuberculosis and Respiratory Diseases
关键词
支原体属
肺炎
聚合酶链反应
诊断
Mycoplasma Mycoplasma pneu-moniae Polymerase chain reaction
