摘要
目的:研究Polo样激酶1(Polo-like kinase 1,PLK1)在胃癌中的表达与各临床病理特征、抑癌基因和肿瘤增殖活性的关系,探讨PLK1在胃癌发生发展中的作用以及其临床意义. 方法:运用免疫组织化学的方法检测正常胃组织15例, 非典型增生组织11例,胃癌54例中PLK1,Ki67, P53的表达. 结果:PLK1在正常胃组织中表达均为阴性;11例非典型增生组织有4例弱阳性;胃癌中有48例阳性(88.9%), 其表达与组织分化程度、远处转移和淋巴结转移无关(P>0.05),与浸润深度(x2=6.775,P<0.01)和临床分期(x2=9.009,P<0.01)相关.在胃癌中,P53阳性38例(70.4%),PLK1的表达与P53蛋白积聚相关(x2=6.664, P<0.05).胃癌中Ki67标记指数均值为34.7±13.4%, 范围为10.3-60.1%,PLK1的表达与Ki67呈正相关(r=0.720,P<0.01). 结论:PLK1在胃癌中高表达,与肿瘤的增殖活性和抑癌基因相关,在胃癌发生发展中起重要作用.
AIM: To investigate the expression of Polo-like kinase 1 (PLK1) and its relationship with clinicopathological characteristics, anti-oncogene and tumor proliferation in human gastric carcinoma, and to explore the role of PLK1 in the carcinogenesis and progression of tumor and its clinical significance. METHODS: The expression of PLK1, P53 and Ki67 was detected in tissues of gastric carcinoma (n = 54), atypical hyperplasia (n = 10)and normal gastric mucosa (n = 15) by immunohistochemical method. RESULTS: PLK1 was negatively expressed in normal mucosa. Weakly positive staining for PLK1 was observed in 4 out of 11 Atypical hyperplasia tissues. The expression of PLK1 was elevated in 88.9%(48/54) of the gastric carcinoma. There were no significant associations between PLK1 and clinicopathological characteristics such as his-tological differentiation, distant metastasis and lymph node metastasis (P>0.05). PLK1 expression was significantly related to the depth of invasion (x2= 6.775, P<0.01) and TMN staging (x2=9.009, P<0.01). In gastric cancer, positive staining for P53 was detected in 38 of 54 cases (70.4%). P53 expression was significantly associated with PLK1 (x2 = 6.664, P<0.05). The mean value of Ki67 labelling index (Ki67 LI) was 34.7 ± 13.4%, with a range of 10.3-60.1%. PLK1 expression was positively associated with Ki67 level (r= 0.720, P<0.01). CONCLUSION: PLK1 is over-expressed in gastric cancer, and associated with tumor proliferation and anti-oncogene. PLK1 plays an important role in the carcinogenesis and development of gastric carcinoma.
出处
《世界华人消化杂志》
CAS
北大核心
2005年第13期1499-1502,共4页
World Chinese Journal of Digestology
