摘要
应用人乳头瘤病毒（ＨＰＶ）通用引物介导的聚合酶链反应（ＰＣＲ）技术检测了１５例结肠癌石蜡包埋病理组织切片中ＨＰＶＤＮＡ，其中１０例呈阳性扩增（阳性率为６６．７％）。１２例正常结肠组织经上述ＰＣＲ检测均呈阴性反应。阳性扩增产物经核酸斑点杂交进行ＨＰＶ型别分析，ＨＰＶ１６型占４例（４０．０％），１８型１例（１０．０％），１６／１８型５例（５０．０％），未检出其他ＨＰＶ型别。表明ＨＰＶ可能对结肠癌的发生具有病原相关性。
We have examined the occurence of human papillomavirus (HPV) DNA in paraffin section of colon carcinoma by general primermediated polymerase chain reaction (GP-PCR). A total of 15 cases were detected, among which the HPV DNA was amplified from 10 cases (66.7%).No HPV DNA was found in all of the 12 normal colon specimens tested . All the PCR positive sample were further examined by dot-blot analysis for HPV genotypes, Cloned HPV types 6b, 11,16 and 18 specific PCR products were used as probes. Out of the 10 PCR positive cases HPV16, was found in 4 cases (40%), while HPV18 in 1 case(10%), HPV16/18 was found in 5 cases (50%), No additional HPV genotypes were found in this relativelly small tested group.The results showes that HPV infection may play a role in the pathogenes is of colon carcinoma.
出处
《中华实验和临床病毒学杂志》
CSCD
1995年第2期165-167,共3页
Chinese Journal of Experimental and Clinical Virology
关键词
聚合酶链反应
结肠肿瘤
DNA
乳头状瘤病毒
General primer-mediated polymerase chain reaction (GP-PCR)
Colon carcinoma
Human papillomavirus
