摘要
对１９８９～１９９２年在湖北省内收集的２４９份婴幼儿腹泻粪便标本用酶联免疫吸附试验（ＥＬＩＳＡ）、碳凝集试验（ＣＡＴ）试剂盒和ＲＮＡ电泳（聚丙烯酰胺凝胶电泳，ＰＡＧＥ）等３种方法检测Ａ组轮状病毒（ＲＶ）。这３种方法各有优点，可基本满足临床检测的需要。对部分阳性样品还用聚合酶链反应（ＰＣＲ）方法进行分型。共检出７种电泳型，３种血清型的Ａ组ＲＶ，另有１种毒株经ＰＣＲ鉴定型别特殊，有待进一步研究。在样品收集期间，有多种电泳型和血清型同时或交替流行，以血清Ｇ１型占优势，Ｇ２型次之，Ｇ３型较少。Ａ组ＲＶ的遗传多样性增加了预防和控制的难度。
The 249 fecal samples obtained from the children with diarrhea in Hubei province during 1989-1992 were tested for group A rotavirus (RV) by enzyme-linked immunosorbent assay (ELISA),carbon agglutination test (CAT) and RNA electrophoresis. Each of these three methods had its advantage and could meet the requirements of the clinical diagnosis test.Some of the RV-positive samples were furthur typed by polymerase chain reaction (PCR). Seven electropherotypes and three serotypes of group A RV were detected. It was shown that in this period serotype G1 was predominant. G2 was second and G3 was only occasionally detectable. The genetic variation of RV made it harder to control this virus.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
1995年第3期250-253,共4页
Chinese Journal of Experimental and Clinical Virology
关键词
婴幼儿腹泻
轮状病毒
流行病学
分子流行病学
Rotavirus, RNA electrophoresis, Serotype, Polymerase chain reaction typing.
