摘要
分离ＳＤ大鼠肝细胞，负载荧光钙指示剂Ｆｕｒａ－２／ＡＭ后以无钙Ｈａｎｋｓ液悬浮，分别加入以下试剂：１．二甲亚砜。２．环孢素（ＣｓＡ）１ｍｇ／Ｌ。３．ＣｓＡ１０ｍｇ／Ｌ。４．ＣｓＡ１０ｍｇ／Ｌ，山莨菪碱１０ｍｇ／Ｌ。５．ＣｓＡ１０ｍｇ／Ｌ，山莨菪碱４０ｍｇ／Ｌ。测定肝细胞内游离钙发现ＣｓＡ可增加肝细胞内游高钙，为剂量依赖性。山莨菪碱可部分拮抗ＣｓＡ引起的升钙作用。
Hepatocytes were prepared from SD rat.After loaded with
fluorescent Ca￣(++) indicatorFura -2/AM , the cells were suspended in Cafree Hanks’
medium. Various agents wereadded into the medium respectively:DMSO,CsA 1μg/ml,CsA 10μ
g/ml,CsA 10μg/ml,anisodamine 10μg/ml, CsA 10μg/ml andanisodamine 40μg/ml. The
cytosolic free Cawere measured and the results showed thatCsA increased the cytosolix free
Ca￣(++) con-tent, while anisodamine could partly antago-nize the effect.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
1995年第6期360-361,共2页
Chinese Journal of Experimental Surgery
基金
上海市科学技术发展基金
关键词
环孢素
山莨菪碱
肝细胞
钙
毒理学
CsA,anisodamine , cytosolicfree Ca￣(++) ,hepatocytes
