摘要
为证实乙型肝炎（乙肝）血清学标志酶联免疫吸附试验（ＥＬＩＳＡ）试剂中五种抗原抗体反应的特异性，采用排列组合的交叉反应方式，对Ａｂｂｏｔｔ和部分国产试剂盒中五种抗原抗体进行交叉反应研究，结果表明：所有生产商的乙肝血清学标志ＥＬＩＳＡ试剂盒中，酶标记抗－ＨＢｅ和包被的ＨＢｃＡｇ都存在交叉反应，大部分生产商的抗－ＨＢｃ与ＨＢｅＡｇ也存在交叉反应。实验证实抗－ＨＢｅ和抗－ＨＢｃ之间存在非恃异性交叉反应。考虑临床上抗－ＨＢｃ和抗－ＨＢｅ检测阳性率较高，非特异性交叉反应可能是重要原因。
n order to examine the specificities of the antigen and antibody regents used in kits for serological detection of HBV the five antigens and antibodies of Abbott and some home-made HBV ELISA kits were tested for cross-reactions between them by Chess-board titrations. The study showed that cross re-action between anti-HBe and HBcAg was found in the test kits from all companies. Cross reactions be-tween anti-HBc and HBeAg were found in most kits.The non-specific cross reactions of anti-HBe and anti-HBc comfirmed in this study, may be an important reason to explain the higher positive rate in us-ing such anti-HBe and anti-HBc.
