摘要
建立测定乙肝病毒脱氧核糖核酸（ＨＢＶＤＮＡ）的聚合酶链反应－溴乙锭法（ＰＣＲ－ＥＢ），该方法特异性好，灵敏度为１ｐｇＨＢＶＤＮＡ，已能检出ＨＢＶ敏感动物黑猩猩的的最小感染量。应用ＰＣＲ－ＥＢ法测定氯消毒剂对ＨＢＶ的灭活作用表明，有效氯１２５０ｍｇ／Ｌ作用６０分钟或２５００ｍｇ／Ｌ作用３０分钟可使１０倍ＰＣＲ灵敏度的纯化ＨＶＢＤＮＡ转阴；有效氯２５００ｍｇ／Ｌ作用３０分钟以上可使１０倍ＰＣＲ灵敏度的纯化Ｄａｎｅ颗粒中ＨＢＶＤＮＡ转阴；有效氯６２５ｍｇ／Ｌ作用３０分钟或１２５０ｍｇ／Ｌ作用１０分钟可使１０倍ＰＣＲ灵敏度的纯化Ｄａｎｅ颗粒上ＨＢｓＡｇ转阴。因此认为，ＰＣＲ用于评价氯消毒剂对ＨＢＶ的灭活效果是可行的；ＨＢＶＤＮＡ作为ＨＢＶ的灭活指标比ＨＢＶ表面抗原更为直接可靠。
olymerase chain reaction-ethidium bromide(PCR-EB)method for detecting hepatitis B virus DNA (HBV-DNA)was established with good specifictiy and a detection limit of 1 pg HBV-DNA,mini- mum HBV infection dose in susceptible animal, chimpanzees,could be detected with it.Determination of inactivation of HBV-DNA cluld be inactivated with active chlorine 1250 mg/L for 60 minutes,or 2 500 mg/L for 30 minutes,10 pg HBV-DNA in purified Dane particles could be inactivated by active chlorine 625 mg/L for 10 minutes. Accordingly,use of PCR to evaluate the effects of cholorine disinfec- tant in inactivating HBV was feasible, and HBV-DNA was a more reliable index for inactivation of HBV than HBsAg.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
1995年第3期159-161,共3页
Chinese Journal of Preventive Medicine
关键词
聚合酶链反应
乙型肝炎病毒
消毒剂
Polymerase chain reaction Hepatitis B virus Disinfectants
