摘要
建立培养人胚肝细胞脂质过氧化损伤模型，发现模型组细胞存活率下降，白蛋白分泌减少，丙氨酸转氨酶释放增加，与对照组比较差异有极显著性。电镜下细胞超微结构模糊，膜及膜性细胞器肿胀，或膜性结构断裂。加硒预防组上述损伤变化明显减轻，伴谷胱甘肽过氧化物酶活性增高。加硒治疗组上述保护作用较弱。提示预防性补硒可减轻脂质过氧化对人肝细胞的损害。
selenium protection model of buman fetal hepatocyte in vitro injured by lipid peroxidatin was es- tablished. It was found that survival rate and secretion of albumin of the injured cells decreased, and re- lease of alanine transaminase (ALT)and its activities in the cells increased, as compared with those in controls,with a very significant difference. Under clectronic microscope, ultrastructure of the injured cells appeared obscure,their membtane and membranous organella swelling,or membranous structure breaking. Injuries mentioned above in cells pre-treated with selenium were obviously decreased and ac-tivities of glutathione peroxidase increased.But protective effects of seienium supplempnt were weaker. It suggested that preventive supplement with selenium could reduce injury to human hepatoeyte caused by lipid peroxidation.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
1995年第3期165-167,共3页
Chinese Journal of Preventive Medicine
