摘要
作者研究了两种ｃＡＭＰ类似物，双丁酰ｃＡＭＰ（ＤＢｃＡＭＰ，无位点选择性）和８－Ｃｌ－ｃＡＭＰ（位点选择性）对高转移人肺癌细胞系ＰＧ细胞生长的调控作用。ＭＴＴ法检测结果显示，ＤＢｃＡＭＰ１ｍｍｏｌ／Ｌ在第８天，对ＰＧ细胞生长的抑制率为３０％，而８－Ｃｌ－ｃＡＭＰ１０－２０μＭ为７５％～８０％。磷酸二酯酶抑制剂ＩＢＭＸ与８－Ｃｌ－ｃＡＭＰ合用没有增加其抑制作用，而与ＤＢｃＡＭＰ合用则具有明显协同作用。Ｂｏｙｄｅｍ小室法和软琼脂集落形成实验证明，处理的细胞其浸润能力和集落形成能力降低。放射免疫测定表明，ＤＢｃＡＭＰ处理组细胞内的ｃＡＭＰ水平明显高于８－Ｃｌ－ｃＡＭＰ处理组。结果提示，８－Ｃｌ－ｃＡＭＰ和ＤＢ－ｃＡＭＰ可能通过不同的作用机理影响ＰＧ细胞的生长和浸润。
Abstract Type Ⅰand type Ⅱ cAMP receptor proteins
participaate in cell growth regulation,differentiation and neoplastic
transformation of neoplasms. In this paper the growth
regulatoryeffects of cAMP analogs,DBcAMP(non-site-selective) and
8-Cl-cAMP(site-selective)on ahighly metastatic human lung cancer cell
line PG were studied. By MTT assay,DBcAMP at1mmol / L was found to
inhibit PG growth by 30% on the day 8, while 8-Cl-cAMP at 10～20μ
mol/L inhibited the growth by 75%～80%. When phosphodiesterase
inhibitor isobuty1-1-methy1-xanthine(IBMX)wax added the inhibitory
effect of 8-Cl-cAMP was not enhanced,whereas that ofDBcAMP was
significantly enhanced. The invasiveness and colony-forming ability
of the treat-ed cells decreased. The cAMP Ievel asdetermined by RIA
was much more increased inDBcAMp-treated than in 8-Cl-cAMp-treated PG
cells. The result suggests that 8-Cl-cAMPand DBcAMP exert their
effects on tumor cell growth and invasion by defferent mechanisms.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
1995年第2期108-111,共4页
Chinese Journal of Oncology
