摘要
本研究把Ⅰ型人酪氨酸羟化酶(human tyrosine hydroxylase type Ⅰ;HTH_1)cDNA连接到逆转录病毒载体LNSX上,构建成真核表达载体 LNSHTH_1,然后通过in vivo途径转染帕金森病模型鼠的纹状体细胞。外源的HTH_1基因在宿主脑内得到了表达,并使其病理行为改善约70％。这种新的基因治疗帕金森病的方式,有其独特的优越性。
We constructed an eukaryotic expression plasmid LNSHTH1 by inserting human tyrosin hydroxylase type I (HTH:) cDNA into the retroviral vector LNSX. The recombinant plas mid LNSHTHi was transfected into the striatum of Parkinson's disease (PD)rats in vivo. E pression of the foreign HTH gene was found by TH immunohistochemistry and rotational be havior of the PD rats was amelirated by 70 per cent. This new method of gene therapy for P. offers some unique advantages.
出处
《临床神经科学》
1995年第4期194-196,共3页
Chinese Journal of Clinical Neurosciences
基金
国家自然科学基金(编号39470713)
关键词
基因疗法
基因转移
震颤麻痹
Parkinson's disease Gene therapy Gene transfer
