摘要
角膜上皮细胞的重要性已逐渐为人们所重视,对角膜上皮细胞的基础和临床研究亦日渐深入。角膜上皮细胞的组织培养作为研究角膜上皮细胞的一种很好的技术方法,已被很多实验室广泛应用于角膜上皮细胞的胶原、胶原酶代谢产物,粘蛋白代谢产物,病毒感染的影响,细胞分化,各种生长促进因子和药物对上皮细胞生长的影响。
Corneal buttons denuded endothelium and Descemet's membrane were trypsinizated with 0.25% trypsin in a Hank's BBS at 4℃ for 14-16 hours. Corneal epithelial cells were scraped and cultured on gelatin membrans with plastic ring. Cultured epithelial cells adhered quickly the membranes and grew well in the tissue culture medium supplemented with cholera toxin. Cultured cells reached confluency in 7-10 days, after which they morphologically resembled native corneal epithelium. This system is useful for in vitro studies of pharmacology and toxiology for corneal epithelium.
出处
《眼科研究》
CSCD
1989年第2期72-74,共3页
Chinese Ophthalmic Research
