摘要
目的：鉴定自制的５－羟色胺（５－ＨＴ）抗独特型抗体，初步了解５－ＨＴ受体在哺乳动物体内的定位．方法：用免疫组织化学ＡＢＣ法．结果：待测的５－ＨＴ抗独特型抗体既能和５－ＨＴ抗体也能和５－ＨＴ受体发生特异性结合．它能使大鼠胃底腺的壁细胞和主细胞、胃底的平滑肌细胞、胰的内外分泌细胞、第三脑室的室管膜上皮细胞、侧脑室脉络丛上皮细胞、舌下神经核的神经元、结肠癌细胞及血小板都显示为５－ＨＴ受体免疫反应阳性．这些细胞除了胃底腺的主细胞外都已被一些学者用功能受体法或放射自显影法证明含有５－ＨＴ受体而且主要含有５－ＨＴ＿１和５－ＨＴ＿２受体．结论：待测５－ＨＴ抗独特型抗体为β型５－ＨＴ抗独特型抗体（５－ＨＴａｂ＿２β）．它主要能识别５－ＨＴ＿１，和５－ＨＴ＿２受体．
Objective:To identify the homemade anti-idiotypic antibody and to achieve an initial understanding of the localization of serotonin receptors in mammals. Methods:The immunohistochemical ABC method was employed.Results:The homemade antiserotonin anti-idiotypic antibody combined with both serotonin anti-body and serotonin receptors.It could cause 5-HT receptor positive immunoreactions in the chief cells and parietal cells of the rat gastric gland, smooth muscle cells of the rat stomach fundus and of the small veins of the rat stomach submucosa,endocrine and exocrine cells of the rat pancreas,ependymal cells of third ventri-cle,epithelial cells of choroid plexus of lateral ventricle in the rat brain,neurons in the hypoglossal nucleus,human colonic cancer cells and rat platelets.These cells,all except the chief cells in the gastric gland,have been proved by some scholars using functinal receptor technique and autoradiography to contain serotonin re-ceptors,most of which are receptors of 5-HT_1 and 5-HT_2. Conclusion:Homemade serotonin is βtype sero-tonin anti-idiotypic antibody(5-HTab_2β)and mainly acts to recognize the receptors of both 5-HT_1 and 5-HT_2.
出处
《第四军医大学学报》
1995年第3期195-198,共4页
Journal of the Fourth Military Medical University
关键词
5-羟色胺
抗独特型抗体
受体定位
免疫组织化学
serotonin
anti-idiotypic antibody
receptor localization
immunohistochemistry
