摘要
目的：探讨辐射增敏剂ＡＫ２１２３和辐射防护剂ＷＲ２７２１对肝癌细胞ＤＮＡ损伤与修复的影响．方法：用脉冲交变电泳法（ＰＦＧＥ）和￣３Ｈ－ＴｄＲ掺入法，测定上述两药物对受照人肝癌细胞ＤＮＡ双链断裂（ｄｓｂ）与修复及ＤＮＡ合成的影响．结果：在０～８０Ｇｙ剂量范围内，随着照射剂量增加，肝癌细胞ＤＮＡ残留率逐渐降低（１００％～５７．０％），ＤＮＡｄｓｂ量亦逐渐增多，照前给ＡＫ２１２３或ＷＲ２７２１，均能显著地降低ＤＮＡ残留率，使ＤＮＡｄｓｂ量增多，加重ＤＮＡ损伤（Ｐ＜０．０５）；２０Ｇｙ受照细胞保温０～１２０ｍｉｎ，在６０ｍｉｎ时ＤＮＡｄｓｂ开始被修复（Ｐ＜０．０５），而ＡＫ２１２３组为１２０ｍｉｎ（Ｐ＜０．０５），ＷＲ２７２１在３０ｍｉｎ后有修复的趋势；ＡＫ２１２３在６２．５～５００μｇ／ｍｌ内其参入率显著升高（Ｐ＜０．０５），呈促进ＤＮＡ合成作用，ＷＲ２７２１则随药物浓度增加其参入率降低（Ｐ＜０．０５），抑制ＤＮＡ合成．结论：ＡＫ２１２３和ＷＲ２７２１显著加重受照肝癌细胞的辐射损伤，促进ＤＮＡｄｓｂ量增加，抑制其修复过程．
Objective:The effects of radiation-sensitizing agent AK2123 and radiation-protective agent WR2721 on DNA double strand break (dsb ),its restoration and DNA synthesis in irradiated human hepato-carcinoma cells were determined. Method : Pulsed field gel electrophresis (PFGE)and ￣3H-TdR endosmosis were used, Results :Within the range of 0～80 Gy,the increase of irradiation dosage resulted in a gradual de-crease of DNA residual rate of hepatocarcinoma cells(100%～57.0%)or a gradual increase of DNAdsb.Ad-ministration of AK2123 or WR2721 prior to irradiation could markedly decrease DNA residual rate,promate the production of DNAdsb and worsen DNA damage (P<0. 05);irradiated cells by 20 Gy were kept at 37℃for 0～120 min,60 min later,DNAdsb began to restore(P<0.05),whereas restoration occurred in AK2123group 120 min later(P<0.05),and 30 min had passed before DNAdsb tended to restore in WR2721 group;with its concentration ranging 62.5～500μg/ml,the endosmotic rate of AK2123 registered a significant rise(P<0.05),indicating a promotion of DNA synthesis,while WR2721,with the increase of its concentration,had a decreased endosmotic rate(P<0.05),indicating an inhibition of DNA synthesis.Conclusion: All the above results suggest that AK2123 and WR2721 can remarkably aggravate radiation damage of the irradiated cells,promate the production of DNAdsb and inhibit its restoration.
出处
《第四军医大学学报》
1995年第4期266-268,共3页
Journal of the Fourth Military Medical University
关键词
肝脏肿瘤
放射疗法
辐射增敏剂
辐射防护剂
DNA
radiation-sensitizing agents
radiation-protective agents
pulsed field gel electrophresis
DNA double strand break
ionizing radiation
