摘要
从古代生物中获取的古代ＤＮＡ序列为进一步从分子水平上认识古代生命及其演化提供了新的证据。分析和甄别古代ＤＮＡ序列是古代ＤＮＡ研究的关键步骤之一。本文着重讨论甄别古代ＤＮＡ序列的主要标准如：野外取样与实验室控制、ＤＮＡ“行为”分析、系统发育验证和实验结果的可重复性，并在此基础上评述近期发表的有关恐龙ＤＮＡ的研究。安成才等（１９９５）发表了６条来自一枚恐龙蛋化石中的１８ＳｒＤＮＡ序列，但未做进一步的系统发育研究。本文按１８ＳＲＮＡ分子的二级结构模式对已发表的恐龙蛋ＤＮＡ序列与现生生物各类群代表的１８ＳｒＤＮＡ序列进行了重新对位排列并做了系统发育分析。所获结果表明原文中的两组１８ＳｒＤＮＡ序列分别来自于至少两种不同的生物，它们在系统树上的位置均不在脊椎动物分支之内，其中一组序列同现生的高等植物呈姊妹群关系。
Studies of ancient DNA from archaeological and paleontological material have provided new insight into our understanding of ancient life and molecular evolution over a largetime span. Because of the sensitivity of the PCR technique used for ancient DNA amplification, authentication of target DNA has become a serious consideration for ancient DNAworkers. This paper discusses the current criteria (e. g. laboratory control,DNA behavior,phylogenetic test, and reproducible experiments)(Text-fig.1) for establishing authentication of a putative ancient DNA sequence. Based on these authenticating criteria,a reassessment was made for recently published DNA sequences which were claimed to have been derived from a Late Cretaceous dinosaur egg.
出处
《古生物学报》
CAS
CSCD
北大核心
1995年第6期657-673,共17页
Acta Palaeontologica Sinica
关键词
古代DNA
序列甄别
恐龙蛋
DNA
Ancient DNA
Authentication
18S rDNA
Dinosaur egg
Alignment
Phylogeny
