摘要
本文介绍性病淋球菌ＰＣＲ基因检测方法的研究过程．笔者设计一对对淋球菌隐蔽性质粒ｃｐｐＢ基因有专一性的引物，应用该引物进行ＰＣＲ体外基因扩增．对淋病患者的泌尿生殖道标本可检出３８０ｂＰ特异片段应用该试剂盒检测１２５例性混乱患者与细菌培养法比较，前者检出率２５．６％（３２／１２５），后者仅６．４％（８／１２５）．
This paper discusses the method of the gene amplification of polymerase chain reaction for Neisseria gonorrhea.The author has designed a pair of primers for the cppB gene of the cryptic plasmid of Neisseria gonorrhea, which amplificated a 380 base pair specific fragment for detecting the urogenital samples of gonorrhea patients.125 sex-disturbance women have been examined by the reagent kit and the cultured method,respectively.By contrast,32 out of 125(25.6%) show positive results by the polymerase chain reaction and only 8 out of 125(6.4 %)show positive results by the cultured method.
出处
《海南大学学报（自然科学版）》
CAS
1995年第2期138-141,共4页
Natural Science Journal of Hainan University
基金
海南省卫生厅1992年度科研基金
关键词
淋球菌
多聚酶链反应
基因扩增
检测
性病
Neisseria gonorrhea
polymerase chain reaction
gene amplification
