摘要
用异硫氢酸标记牛血清白蛋白，过柱后荧光蛋白浓度与荧光值呈明显正相关（ｒ＝０．９９，Ｐ＜０．０１）．大鼠颈外静脉注射荧光蛋白后１ｈ，取心肌匀浆，测定其荧光值。本法简便易行，重复性好，为观察在体心脏微血管壁通透性提供了一种新的方法。
new method for the determination of myocardial microvascular permeability in rats INVIVO was
establishe.Bovine serum albumin was labelled with fluorescein isothiocyanate and in-jeced into
external juglar vein. After 1h, the heart was removed and homogenated. The intensityof
fluorescence was determined. The concentration of fluorescein-albumin was correlated with
theintensity of fluorescein(r=0.99,P<0.01).This method is simple and efficient.
出处
《基础医学与临床》
CSCD
1995年第4期77-79,共3页
Basic and Clinical Medicine
基金
国家自然科学基金
关键词
心脏
微血管壁
通透性
测定方法
异硫氢酸荧光素
fluorescein isothiocyanate microvascular permeability heart rats Departiment of
Pathophysiology,Shanghai 200433
