摘要
目的探讨酒精对大鼠睾丸诱导型一氧化氮合酶(iNOS)、Bcl-2基因表达和生精细胞凋亡的影响。方法30只成年健康SD雄性大鼠随机均分为对照组、低剂量组和高剂量组,用不同剂量的酒精灌胃成年大鼠26d(两个生精周期)后,免疫组织化学法(SP法)检测睾丸iNOS、Bcl-2基因表达的变化;原位缺口末端标记法(TUNEL法)检测细胞凋亡指数(A1)的变化。结果与对照组相比,低剂量组大鼠睾丸iNOS、Bcl-2基因表达强度和细胞凋亡指数(A1)无明显变化(P>0.05);而高剂量组与对照组和低剂量组相比,iNOS表达显著增强(P<0.01),Bcl-2基因表达明显减弱(P<0.01,P<0.05),细胞凋亡指数则增加(P<0.01)。结论长期大量饮酒可以诱导睾丸生精细胞凋亡增加,iNOS与Bcl-2基因表达的改变是重要原因之一。
Objective To investigate tha alterations of iNOS, Bcl-2 and the spermatogenic cells apoptosis in the rat testis submitted to alcohol. Methods Thirty healthy male Sprague-Davwley rats were randomly divided equally into three groups： control group, low dose group and high dose group. Alcohol was administered intragastrically at different doses to three groups of adult rats respectively. After twenty-six days, the immunohistochemical staining method （SP method） for detecting the expression of iNOS and Bcl-2 in the testis and TUNEL （TdT-mediated dUTP-X nick end labeling） method for detecting AI （apoptotic index） were used in the present study. Results Compared with control group, no significant differences were noted in the expression of iNOS, Bcl-2 and AI in low dosegroup （P〉0.05）; but the expression of iNOS increased significantly （P〈0.01） and that of Bcl-2 decreased markedly （P〈0.05, P〈0.01 ）, the AI of spermatogenic cells in high dose group was predominantly higher than that in low dose and control group （P〈0.01）. Conclusion The expression variations of iNOS and Bcl-2 is one of main causes that accohol overtaking induces spermatogenic ceils apoptosis.
出处
《中国男科学杂志》
CAS
CSCD
2005年第4期8-11,共4页
Chinese Journal of Andrology
