摘要
从福建省推广的谷杆两用水稻201中分离出内生细菌12株,鉴定为阴沟肠杆菌(Enterobactercloacae)、鹑鸡肠球菌(Enterococcusgallinarum)、短小芽孢杆菌(Bacilluspumilus)、地衣芽孢杆菌(Bacilluslicheniformis)、巨大芽孢杆菌(Bacillusmegaterium)、球形芽孢杆菌(Bacillussphaericus)、蜡状芽孢杆菌(Bacilluscereus)、脂肪杆菌(Pimelobacter)和节杆菌(Arthrobacter)。选择固氮酶活性较大的3个菌株,对其抗性、革蓝氏染色特性进行了研究。采用三亲交配法将标记基因nifH-lacZ导入到鹑鸡肠球菌中,用该固氮细菌回接水稻,对感染鹑鸡肠球菌的水稻根进行β-半乳糖苷酶组织化学染色、光学显微镜和电子显微镜观察。结果表明,固氮细菌鹑鸡肠球菌在水稻根表皮细胞、内皮层细胞、维管组织细胞和细胞间隙中存在。
Twelve strains of endophytic diazotrophic bacteria were isolated from grain-straw-dual-purpose rice 201 popularized in Fujian Province. They were identified as： Enterobaeter cloacae, Enterococcus gallinarum, Bacillus purnilus, B. licheniformis, B. megaterium, B. sphaericus, B. cereus, Pirnelobacter, and Arthrobacter. Three strains with high acetylene reduced activity were selected to study the bacterial resistance to antibiotics, and results of Gram staining. The plasmids harboring the reporter gene lacZ fused to the promoters of nifH were transformed into Enterococcus gallinarum by triparental crossing. The inoculated rice roots were examined by β-galactosidase staining and observed under light and electron microscopes. The results indicated that the endophytic diazotrophic bacteria existed in epidermal cells, cortex cells, vascular tissue cells and intercellular spaces office roots.
出处
《热带亚热带植物学报》
CAS
CSCD
北大核心
2005年第4期296-302,共7页
Journal of Tropical and Subtropical Botany
基金
国家自然科学基金项目(30070467)
福建省科技厅青年人才创新项目(2001J047)
福建省博士后基金项目资助
关键词
水稻
内生固氮细菌
分离
分布
Rice
Endophytic diazotroph
Isolation
Distribution
