摘要
以明星肉鸡和丝毛乌骨鸡杂交产生的F2资源群体为试验材料,根据鸡载脂蛋白AI(Apo-AI)基因的5′端序列设计引物,采用测序和PCR-SSCP方法进行SNP检测和基因型分析,探讨Apo-AI基因多态性与鸡生长和体组成性状之间的关系。研究发现在Apo-AI基因序列起始密码子ATG上游163bp处存在一个A/T突变。该突变产生的不同基因型与鸡生长和体组成性状进行的统计分析结果表明,BB基因型个体的1、2周龄体质量显著高于AA和AB基因型个体的相应体质量(P<0.05);BB基因型个体腹脂质量显著高于AA基因型个体的腹脂质量(P<0.05);BB和AB基因型个体的腹脂率显著高于AA基因型个体的腹脂率(P<0.05)。可以尝试将Apo-AI基因应用于鸡生长和腹脂性状的分子标记辅助选择育种方案中。
In present study, a F2 population derived from Broiler crossing to Silky was used to investigate the effect of Apolipoprotein AI （Apo-AI） gene on chicken growth and body composition traits. Primers for 5＇ region of Apo-AI gene were designed according to chicken genomic sequence from database. Polymorphism within the region was detected by DNA sequencing, and PCR-SSCP method was then developed to screen the F2 population. An A / T single nucleotide polyporphism （SNP） at base 163 （GenBank accession No. M96012） upstream of the ATG initiation codon of Apo-AI gene was found. The statistical results showed that the Apo-AI-BB genotype birds had a higher body weight of 1 and 2 weeks of age than the birds with Apo-AI-AB and Apo-AI-AA genotypes （P〈0. 05） ; the Apo-AI-BB genotype birds had a higher abdominal fat weight than the birds with Apo-AI-AA genotype （P〈0. 05） ; the Apo-AI-BB and Apo-AI-AB genotypes birds had a higher abdominal fat percentage than the birds with Apo-A[-AA genotype （P〈0.05）. The Apo-AI gene is, therefore, a potential marker for use in molecular marker-asslsted selection program related with growth and abdominal fat traits.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2005年第8期751-754,共4页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
"863"国家高技术研究发展计划(2002AA211021)
国家自然科学基金(30270950)
教育部优秀青年教师资助计划项目(1985)
