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不同贮藏温度和时间对钻喙兰人工种子萌发及其幼苗成活的影响 被引量:8

Effects of storage temperature and time on germination of synthetic seeds and survival of seedlings of Rhynchostylis retusa
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摘要 Seeds of Rhynchostylis retusa (L.) Bl. germinate faster on the KC medium than on the VW medium. Protocorms of R. retusa were successfully encapsulated in the KC solution (containing 3% sodium alginate) to get synthetic seeds and germination rate of synthetic seeds stored at 4℃ was higher than at 20℃ and 25℃ on the KC medium containing 15% coconut milk, 2 g·L -1 peptone and 1 mg·L -1 NAA. Time taken for germination will be longer and germination rate will decrease at different storage temperatures with the increasing of storage time. Survival rates of seedlings from the synthetic seeds stored at 4℃ for 15, 30, 45, 60 and 75 d are 93%, 89%, 80%, 75% and 63% on field transfer, respectively. Seeds of Rhynchostylis retusa ( L. ) B1. germinate faster on the KC medium than on the VW medium. Protocorms of R. retusa were successfully encapsulated in the KC solution (containing 3% sodium alginate) to get synthetic seeds and germination rate of synthetic seeds stored at 4℃ was higher than at 20℃ and 25℃ on the KC medium containing 15% coconut milk, 2 g·L^-1 peptone and 1 mg·L^-1 NAA. Time taken for germination will be longer and germination rate will decrease at different storage temperatures with the increasing of storage time. Survival rates of seedlings from the synthetic seeds stored at 4℃ for 15, 30, 45, 60 and 75 d are 93% , 89% , 80% , 75% and 63% on field transfer, respectively.
机构地区 上海植物园
出处 《植物资源与环境学报》 CAS CSCD 2005年第3期63-64,共2页 Journal of Plant Resources and Environment
关键词 钻喙兰 人工种子 贮藏 萌发率 成活率 Rhynchostylis retusa (L.) Bl. synthetic seed storage germination rate survival rate
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参考文献4

  • 1Singh F. Encapsulation of Spathoglottis plicata protocorms [J].Lindleyana, 1991, 6:61 -62.
  • 2Sharma A, Tandon P, Anjani K. Regeneration of Dendrobium wardianum from synthetic seeds [ J ]. Indian Journal of Experimental Biology, 1992, 30:747 - 748.
  • 3Corrie S, Tandon P. Propagation of Gymbidium giganteum through high frequency conservation of encapsulated protocorms under invivo and in vitro conditions [ J ]. Indian Journal of Experimental Biology, 1993, 31:61 -64.
  • 4Malemnganba H, Ray B K, Bhattacharyya S, et al. Regeneration of encapsulated protocorms of Phaius tankervilliae stored at low temperature [ J]. Indian Journal of Experimental Biology, 1996,34 : 802 - 805.

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