摘要
目的:建立石榴皮中没食子酸定性定量的方法。方法:用薄层色谱法对石榴皮有效成分没食子酸进行定性分析,采用高效液相色谱法测定了没食子酸含量。以DiamonsilC18柱为固定相,流动相0.2%甲醇乙腈0.1%磷酸和0.1%三乙胺水溶液(3∶97),检测波长216nm,柱温40℃,结果:没食子酸在0.085～0.768μg峰面积与进样量呈良好的线性,r=0.9996;平均回收率96.7%;RSD0.8%。结论:通过20批不同产地石榴皮的鉴别和含量测定,证明方法灵敏准确可靠,重复性好。
Objective： To establish the qualitative and quantitative methods of gallic acid in pomegranate rind. Method： The thin layer chromatographic method was used for identitication, and the high performance liquid chromatographic method was used for assay. Extracts were separated on a Diamonsil C18 column eluted with the mobile phase of a mixture of acetonitrile containing 0.2% methanol and water containing 0.1% phosphoric acid and 0.1% TEA （3：97）. The detection wavelength was set at 216 nm, and colum temperature was 40℃. Result： The calibration curve was linear in the range of 0. 085-0.768μg（r=0.9996）. The average recovery was 96.7% （RSD 0.8 %, n=5）.20 batches of the crude drug were identified and assayed，with the methods. Conclusion： The methods were sensitive and reliable, and can be used for quality control of the pomegranate rind.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2005年第15期1171-1172,共2页
China Journal of Chinese Materia Medica
关键词
石榴皮
没食子酸
TLC
HPLC
pomegranate rind
gallic acid
TLC
HPLC
identification
assay
