摘要
采用连续监测法测定以ＮＡＤＰ为辅酶的异柠檬酸脱氢酶（ＩＣＤ），并对酶促反应条件进行了研究。以Ｔｒｉｓ－ＨＣｌ为缓冲体系，酶促反应最适ｐＨ为７．５，Ｍｎ（２＋）为２ｍｍｏｌ／Ｌ，在最佳条件下测定ＩＣＤ的线性范围为０～８５Ｕ／Ｌ，批内变异系数２．０６％～５．８５％，批间变异系数为３．７１％～８．７２％，１００例健康人血清参考范围为２．０～１１．２Ｕ／Ｌ，初步临床应用表明各类肝炎及肝癌均显著升高，ＩＣＤ／ＡＬＴ比值对肝癌的鉴别诊断有一定的应用价值。
A kinetic assay of serum isocitrate dehydroenase(ICD) with NADP was reported in this paper. Optimized reagent and react condition were studied.Optimum pH was at 7.5 for Tris-HCl buffer.and Mn(2+) was 2mmol/L.The linear range is from 0 to 85 U/L.Within and between batch CV are 2.06%～5.85% and 3.71%～8.72%. Serum ICD in 100 healthy subjects was 2.0～11.2U/L.The clinical application of the test proved that it is useful to differential diagnosis of hepatic cancer.
出处
《临床检验杂志》
CAS
CSCD
北大核心
1995年第4期171-173,共3页
Chinese Journal of Clinical Laboratory Science
关键词
连续监测
异柠檬酸脱氢酶
肝炎
肺瘤
血清
Kinetic assay Isocitrate Dehydrogenase Hepatitis Hepatic cancer Serum
