摘要
超氧化物歧化酶，简称ＳＯＤ（Ｓｕｐｅｒｏｘｉｄｅｄｉｓｍｕｔａｓｅ），是一种应用价值大、广泛存在于生物体细胞内的金属酶．本实验以酿酒酵母和嗜热脂肪芽孢杆菌为出发菌株，进行抗Ｈ_２Ｏ_２性能的筛选，选出耐受性好的菌株经２Ｌ发酵罐发酵后，提取到了热稳定性好的Ｃｕ，Ｚｎ－ＳＯＤ和Ｆｅ－ＳＯＤ，酶活力为３２９２和３５４７ｕ／ｍＬ，其比活力为３４５７和３０８４ｕ／ｍｇ．
Superoxide dismutase(abbreviation SOD)are highly-valued metal enzymes which exist in most cell of organisms.The Saccharomyces Cerevisiae and Bacillus Stearothermophilus are used as primitive strains to screen.good strains with high H_2O_2 concentration resistance,which are cultivated in 2L fermentor,extracting Cu,Zn-SOD and Fe-SOD with fine heat stability.The enzyme activity of Cu,Zn-SOD and Fe-SOD are 3292 and 3547u/mL,the specific activity of them are 3457 and 3084 u/mg protein respectively.
出处
《四川师范大学学报（自然科学版）》
CAS
CSCD
1995年第5期6-9,共4页
Journal of Sichuan Normal University（Natural Science）
关键词
酿酒酵母
SOD
微生物
Saccharomyces Cerevisiae
Bacillus Stearothermophilus
Superoxide dismutase
Extracting
