摘要
具有杀虫活性的苏云金芽孢杆菌(B. thuringensis subsp kurstaki)Bt-3701与具有解磷活性的巨大芽孢杆菌(B. megaterium var. phosphaticum)Bm-107原生质体进行了融合。Bt-3701原生质体形成率为99.4％,再生率为21.4％;Bm-107原生质体形成率为97.5％,再生率为23.8％。以PEG为助融剂进行原生质体融合,得到22个融合子,融合率达6.03×10^(-3)。融合子在双抗培养基(DR-CNB)上连续传代12次,得到4个稳定的融合于生物测定表明,融合子既具有一定的杀虫活性又具有一定的解磷活性。
The results of the interspecific protoplast fusion between B. thuringensis sub. kurstaki Bt-3701 which has pesticide ability, and B. megaterium var. phospha-ticum Bm-107 which has decomposing phosphate activity, were reported. High frequency of protoplast formation and regeneration was obtained with 4h activated Bm-107 treated by 100μg/ml lysozyme, and with 2h activated Bt-3701 treated by 3% glycin and mild temperature. Using 40% PEG and 5% nascent Ca2+ to treat the parential protoplast mixture for 3min at 37℃, 4 stable fusants were obtained. Biological tests show that they have both pesticide ability and decomposing phosphate activity, but which are weaker than that of parential strains.
出处
《微生物学报》
CAS
CSCD
北大核心
1995年第5期322-326,共5页
Acta Microbiologica Sinica
关键词
苏云金芽孢杆菌
巨大芽孢杆菌
原生质体融合
Bacillus thuringensis sub. kurstaki, Bacillus megatertum var. phospha- ticum, Protoplast fusion, Pesticide ability, Decomposing phosphate activity
