摘要
用０．５％Ｋ２ＣＯ３溶液提取Ｎ·ｂ、ＳＣＭ６、ＳＣＭ７３种家蚕微孢子虫孢子表面抗原蛋白，并用聚丙烯酰胺凝胶电泳研究了三者表面抗原蛋白的差异，查明三者无共同成份存在。用Ｎ·ｂ抗体蛋白致敏炭素制成检液，对Ｎ·ｂ孢子为阳性反应，对ＳＣＭ６、ＳＣＭ７均不出现阳性凝集反应。可用以有效判别三者中的Ｎ·ｂ孢子。
Spore surface antigen proteins of 3 microsporidians including Nosema bombycis(N.b),Nosema sp.(SCM6) and Endoreticulatus sp,(SCM7) were extracted with 5% K2CO3 solution.And the difference among 3 surface antigen proteins were studied with PAGE.Results revealed that no common bands existed in their PAGE patterns.The N.b spore showed positive reaction to N.br-Ig G sensitized carbon. while SCM6 andSCM7 showed negative reaction, indicating that this method might effectively distinguish the N.b from the SCM6 and SCM7.
出处
《西南农业学报》
CSCD
1995年第3期57-60,共4页
Southwest China Journal of Agricultural Sciences
关键词
家蚕
微粒子
表面抗原
微孢子虫
Bombyx mori L.Microsporidian Surface antigen
