摘要
为进一步评价补充维生素Ｅ和硒的安全性，本文以正常仓鼠幼肾成纤维细细胞株（ＢＨＫ─２１／Ｃ１３）及其多瘤病毒转化的恶性细胞株（ＢＨＫ─２１／ＰｙＹ）为对象，探讨了不同浓度的维生素Ｅ和硒对该对细胞增殖情况的单独和联合作用。结果显示：７μｍｏｌ／Ｌ的维生素Ｅ使ＢＨＫ—２１／ｃ１３和ＢＨＫ—２１／ＰｙＹ细胞株在９６ｈ的细胞计数分别增加１１％和１６％；０．１μｍｏｌ／Ｌ的亚硒酸钠对上述二株细胞的增殖无影响；同时补充以上相同浓度的维生素Ｅ和亚硒酸钠，９６ｈ时的ＢＨＫ─２１／Ｃ１３的细胞计数增长７８％，而ＢＨＫ—２１／ＰｙＹ未改变。提示：低浓度的维生素Ｅ和硒的联合应用，在安全性方面优于二者的单独使用。
In order to evaluate vitamin E and selenium in terms of safety,;We haveimvestigated individual and combined effects of vitamin E and selenium at differentconcentration on proliferation of a normal baby hamster kidney fibroblast cell line(BHK-21/C13) and its polyoma virus transformed counterpart (BHK-21/PyY).The results showed that vitamin E (α-tocopherol), at a concentration of 7μmol/L, stimulated BHK-21/C13 and BHK-21/PyY growth by 11 % and 16% respectively;selenium (sodium selenite), up till to 0. 1μmol/L, had no effect on growthof both cell lines; co-supplementation with vitamin E and selenium at the sameconcentrations as mentioned above increased BHK-21/C13 growth rate by 78%,while BHK-21/PyY cell line remained unaltered. The results suggest that co supplementation with vitamin E and selenium at low concentration is better thanseparate supplementation of them in the light of safety.
出处
《延安大学学报（自然科学版）》
1995年第1期59-63,共5页
Journal of Yan'an University：Natural Science Edition
基金
国家教委优秀青年教师基金
陕西省教委重点科研基金
关键词
维生素E
硒
细胞增殖
恶性细胞
vitamin E selenium cell proliferation tumour.
