摘要
以编码麻风菌(ML)65KD抗原基因的末端重复顺序为扩增靶,建立了检测麻风菌的基因扩增试验,敏感性可达20条菌/μ1,对13株标准分支菌扩增的结果表明特异性也很强。
A PCR method for detection of M. leprae has been established by using the terminal repeated sequence of the gene coded for its 65 KD antigen as the amplification target. It can detect 20 ML/μl and has very strong specificity which had been proved in examination of 13 different strains of mycobacteria.
基金
联合国开发计划署/世界银行/世界卫生组织热带病研究和培训特别规划的资助
IDNo.920048
