摘要
采用反相高效液相色谱法测定复方茵陈注射液中主要成分氯原酸和栀子成的含量。氯原酸在３３５ｎｍ波长测定，栀子成在２３７ｎｍ波长测定。外标法定量，回收率分别为９３．６％和９７．５％。氯原酸和桅子戒回归方程分别为Ａ＝０．１８７＋０．８５８Ｃ（ｒ＝０．９９９３），Ａ＝０．２１＋６．０５５６Ｃ（ｒ＝０．９９８３）。方法操作简单，灵敏度及准确度均较高。
A RP-HPLC method for the determination of chlorogenic acid and gardenoside whichare the main components in Artemisia capillaris Thunb.Compound injection was established.A C_(18) col-wmn and a mobile phase of methanol-water(70:30) were employed.Chlorogenic acid was determinedat 335 nm and gardenoside was determined at 237 nm。 The recovery rate of the two components was93.6%and 97.5%, respectively。For chlorogenic acid,the regression equation was A=0.187 + 0。858C(r=0.9993) and for gardenoside is A=0.21+6.0556C(r=0.9983).The methOd is simple,sensitive and accurate。
出处
《中国药学杂志》
CAS
CSCD
北大核心
1995年第3期166-167,共2页
Chinese Pharmaceutical Journal
