摘要
用狭缝印迹杂交方法在体内、外模型中观察促癌剂对基因表达的影响。结果表明，促癌剂ＴＰＡ可诱导小鼠成纤维细胞ＢＡＬＢ／３Ｔ３及大鼠肝脏中ｃ－ｆｏｓ、ｃ－ｍｙｃ的表达，降低ＢＡＬＢ／３Ｔ３的ＲｂＲＮＡ水平及大鼠肝脏中α１－Ｉ３ＲＮＡ水平。苯巴比妥对大鼠肝脏的长期促癌作用表明，ｃ－ｆｏｓ、ｃ－ｍｙｃ表达有改变，α１－Ｉ３ｍＲＮＡ水平下降。苯巴比妥的短期作用对大鼠肝脏ｃ－ｆｏｓ、ｃ－ｍｙｃ的表达改变不明显，但仍使α１－Ｉ３的表达降低。促癌剂可诱导与肿瘤发生呈正相关的ｃ－ｆｏｓ、ｃ－ｍｙｃ的表达，同时可降低与肿瘤发生呈负相关的Ｒｂ、α１－Ｉ３基因的表达，显示促癌作用在肿瘤形成过程中的重要性，并提示促癌剂可能选择性地对不同性质的基因产生不同的影响。
The modifications of gene expression by tumor promoters were analyzed in vitro and in vivo.The results of slot blot hybridizations showed that tumor promoter TPA induced c-fosand c-myc expressions in mouse fibrdblast cell line BALB/3T3 and rat liver, decreased the 1evels of Rb RNA in BALB/3T3 cell line and of α1-I3 RNA in rat liver,It was also demonstrated that tumor promoter phenobarbital influenced c-fos and c-myc expressions and decreased α1-I3 mRNA level in rat liver during a long term experiment.Phenobarbital was found to have no effect on c-fos and c-myc expressions in rat liver during a short experiment.Tumor promoters induced the expressions of c-fos and c-myc which were positively-related to cancer formation and inhibited the expressions of Rb and α1-I3 which were negatively-related to cancer formation.This implied that tumor promotion played an important role in cancer development and tumor promoters exerted their effects selectively according to the attributes of different genes.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1995年第1期11-15,共5页
Acta Academiae Medicinae Sinicae
基金
国家自然科学基金
"八五"攻关课题基金
关键词
致癌物
促癌作用
基因表达
carcinogens
tumor promotion
gene expression
