摘要
从海南省临高县麻疯树种植基地的枯萎麻疯树树干木质部分离到一病原菌,该病菌从根部入侵,沿木质部导管向上蔓延,最终可导致整株植物萎蔫死亡.根据病害症状特点、病原菌形态特征、培养性状并结合rDNA-ITS区域的序列分析对病原微生物进行了鉴定.结果表明,麻疯树枯萎病的病原菌属半知菌亚门、丛梗孢目、镰孢属、尖孢镰刀菌(Fusarium oxysporum).采用平皿生长速率法测定了多菌灵可湿性粉剂、多菌灵·福美双可湿性粉剂、立枯净可湿性粉剂和代森锰锌可湿性粉剂四种药剂对病原菌的毒力,结果表明多菌灵效果最好,在200μg/mL时,对菌丝的抑制率为98.49%,多菌灵·福美双和立枯净次之,代森锰锌效果较差.
A fungal pathogen was isolated from the infected stem tissue of Jatropha curcas L.growing in Lingao,Hainan,China.The pathogen infected the root at first and then spreaded along with the vessel. And finally,the whole plant completely wilted,even collapsed.The pathogen was identified as Fusariurn oxysporum based on its morphological and culture characteristics,as well as sequence analysis of the internal transcribed spacer(ITS) region of the ribosomal DNA.Screening tests of the fungicides indicated that the effect of 200 μg/mL of carbendazim was the best,whose inhibition rate exceeded 98% ,carbendazim ·thiram and likujing was the second.The worst was mancozeb.
出处
《四川大学学报(自然科学版)》
CAS
CSCD
北大核心
2009年第6期1823-1827,共5页
Journal of Sichuan University(Natural Science Edition)
基金
国家“十一五”科技支撑计划课题(2007BAD50B05)