摘要
目的:采用伊文思蓝荧光的敏感方法,定量分析慢性低灌注脑缺血动物模型正常灌注压恢复过程中血脑屏障通透性的变化规律。方法:实验于2003-12/2004-12在沈阳军区总医院动物实验中心进行。取30只Wistar大鼠,随机分为2组:①对照组(n=5),单纯行右侧颈总动脉和颈外静脉结扎,饲养2周后经尾静脉注入20g/L伊文思蓝生理盐水溶液(4mL/kg),2h后麻醉状态处死。②模型再灌注组(n=25)。采用右侧颈总动脉和颈外静脉端-端吻合,同时结扎上矢状窦,建立大鼠慢性低灌注压性脑缺血的动物模型。饲养2周后阻断右侧颈总动脉和颈外静脉吻合部位形成缺血再灌注,再灌注后2,3,9,24和48h麻醉后处死(每个时间点5只),处死前2h经尾静脉注入与对照组等量的伊文思蓝生理盐水溶液。采用伊文思蓝荧光定量的方法观察各组大鼠血脑屏障通透性的变化。结果:经补充后30只大鼠进入结果分析。再灌注2h时,脑组织伊文思蓝的含量即高于对照组犤(0.935±0.166),(0.489±0.132)mg/g,t=3.872,P<0.05犦,再灌注24h达高峰,再灌注48h趋于平稳犤(5.231±1.183),(5.522±1.124)mg/g犦。结论:在慢性脑缺血正常灌注压恢复过程中血脑屏障通透性的变化规律为,2h增高,24h达高峰,48h趋于平稳。
AIM： To quantificational analyze the change law of permeability of blood-brain barrier during the recovery process of common perfusion pressure in animal models with chronic hypoperfusion cerebral ischemia with the hypersensitive fluorescent evans blue methods. METHODS： The experiment was conducted in Animal Experiment Center of General Hospital of Shenyang Military Area Command from December 2003 to December 2004. Thirty Wistar rats were selected and divided into 2 groups： ① Control group （n=5）, right-sidedness common carotid artery and external jugular vein were deligated purely. Two weeks after raising, the rats were injected with 20 g/L evans blue saline solution （4 mL/kg） through caudal vein, and 2 hours later sentenced to death at drugged state. ② Re-perfusion model group （n=25）： Using an end-to-end anastomosis between the fight carotid artery and the fight external jugular vein and superior sagittal sinus ligation built rat chronic hypoperfusion pressure cerebral isehemia model. The anastomosis part of right-sidedness common carotid artery and external jugular vein performed ischemia re-perfusion. At 2, 3, 9, 24 and 48 hours after re-perfusion and anesthesia the rats were sentenced to death （5 rats at each time point）, and were injected the equivalence evans blue saline solution as the rats in control group 2 hours before death through caudal vein. The changes of blood brain barrier permeability in rats were observed by evans blue fluorescent quantitive methods. RESULTS： After compensation, 30 rats were involved in the result analysis. At 2 hours re-perfusion, the content of evans blue in brain tissue was higher than that in control group [（0.935±0.166）, （0.489±0.132）mg/g, t=3.872, P 〈 0.05]. At 24 hours re-perfusion, it reached the peak, and at 48 hours re-perfusion, it appeared steady [（5.231±1.183）, （5.522±1.124） mg/g]. CONCLUSION： In the recovery process of common perfusion pressure of chronic cerebral ischemia, the change law of permeability of blood-brain barrier was increased at 2 hours, peaked at 24 hours, and 48 hours appeared steady.
出处
《中国临床康复》
CSCD
北大核心
2005年第25期94-95,共2页
Chinese Journal of Clinical Rehabilitation
