摘要
采用聚乙烯醇-硼酸法对WXZ-2、WXZ-8两株菌进行了包埋固定化,通过正交试验优选出较佳包埋条件,即包菌量4%、PVA质量浓度5%、交联时间12 h、小球直径2.5 mm.通过对冷冻干燥,40℃干燥两种保存方式下小球的外观表现、机械强度、微生物活性以及吸附性的测试与比较得出,40℃干燥保存的包埋小球有着更好的活性、机械强度与吸附性能,是一种更理想的干燥保存方式.
Two heterotrophic nitrification aerobic denitrifying bacteria strains,WXZ-2 and WXZ-8,were immobilized by PVA-boric acid method.The optimal embedding conditions were obtained by orthogonal test method.The Quantity of entrapped bacteria was 4%.The concentration of PVA was 5%.The crosslinking time was 12 h.And the diameter of the immobilized beads was 2.5 mm.Two different drying methods,freeze-drying and drying at 40℃,were compared to keep the beads.It was proved that the beads dried at 40℃ showed much better activity,mechanical strength and adsorption performance.
出处
《北京工商大学学报（自然科学版）》
CAS
2012年第1期51-56,共6页
Journal of Beijing Technology and Business University:Natural Science Edition
关键词
包埋固定化
异养硝化
好氧反硝化
正交试验
干燥保存
entrapped immobilization
heterotrophic nitrification
aerobic denitrification
orthogonal test
preservation after drying
