摘要
葛仙米以其资源稀少、营养价值丰富,独特的生物活性和药理作用而引起人们广泛地关注。为探讨葛仙米中的生物活性成分,本文以野生葛仙米藻红蛋白为研究对象,在系统研究葛仙米藻红蛋白的提取、分离和纯化的基础上,采用微弱化学发光法,研究了葛仙米藻红蛋白清除氧自由基的能力。结果表明:清除氧自由基的能力为:粗提物>藻红蛋白>藻蓝蛋白;葛仙米藻红蛋白清除氧自由基的能力为:H2O2自由基的清除率>·OH自由基的清除率>O2·自由基的清除率。
The significant biological and pharmacological activity of Nostoc Sphaeroids kutz with abound nutrition and infrequent distribution had been paid people＇s much attention. In order to ascertain its active component, the phycoerythin was used as material. On the basis of systemically studies on preparation and purification of phycoerythrin, Scavenge capability of oxygen free radical was studied by chemiluminescence. The main results were shown that scavenge capability of oxygen free radical was orderly crud extract, phycoerythrin, phycocynin, and PE was orderly, H2O2 radical, · OH radical, O2^- · radical, respectively.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第8期404-407,共4页
Food Science
基金
湖北省自然科学基金(2003ABA068)
湖北省科技攻关项目(2002A201C15)
湖北省教育厅重大项目(2001A08008)
湖北省优秀中青年科技创新团队计划(鄂科教[2005]3)
关键词
葛仙米
藻红蛋白
体外
抗氧自由基
Nostoc Sphaeroids kutz
phycoerythrin
in vitro
antioxygen free radical activity
