摘要
5－羟甲基糠醛（5－HMF）是糖的降解产物。本文提出了用高效波相色谱法同时测定食品和药品中的蔗糖、葡萄糖、果糖和5－HMF的方法。采用氢型阳离子交换色谱柱AminexHPX－87H，以乙腈－0．01mol／L硫酸（40：60）为流动相分离糖和5－HMF。使用串联的紫外光度检测器（280nm）和示差折光检测器，分别检测5－HMF和糖。
5-Hydroxymethylfurfural (5-HMF) is a degradation product of sugars. A high performance liquid chromatography (HPLC) method for the simultaneous determination of sucrose, glucose, fructose and 5-HMF in foods and pharmaceuticals by HPLC is reported. 5-HMF and sugars are separated on an Aminex HPX-87H column (300× 7. 8 mm) with ace tonitrile-0. 01 mol/L H,SO4 (40: 60) as mobile phase and with dual detectors, a UV detector (280 nm) to measure 5-HMF, and a refractometer to measure sugars.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1996年第1期57-60,共4页
Chinese Journal of Analytical Chemistry
关键词
羟甲基糠醛
蔗糖
葡萄糖
果糖
高效液相色谱
5-Hydroxymethylfurfural
sucrose
glucose
fructose
high-performance liquid chromatography
