摘要
目的:细胞凋亡是实验性变态反应性脑脊髓炎恢复及随后出现缓解的原因之一,观察Wistar大鼠诱发实验性变态反应性脑脊髓炎后,雷公藤甲素的治疗效果和对大鼠脑脊髓中细胞凋亡的影响,并与糖皮质激素地塞米松相比较。方法:实验于2003-03/10在山西大同大学脑科学研究所中心实验室进行。取雌性Wistar大鼠随机分为5组:①地塞米松组(n=10):用抗原匀浆0.4mL/只,双后肢足垫皮下免疫动物,同时足背皮下注射减毒百日咳杆菌0.05mL/只(含5.0×1010个菌体),免疫发病后或免疫后第15天,腹腔注射地塞米松10mg/kg。②模型组(n=10):同前处理,但改为腹腔注射生理盐水0.5mL。③雷公藤甲素组(n=10):同前处理,但改为腹腔注射雷公藤甲素40μg/kg。④完全福氏佐剂组(n=6):用完全福氏佐剂(0.4mL/只)加减毒百日咳杆菌(0.05mL/只)免疫动物,方法同前,免疫后15d腹腔注射生理盐水0.5mL。⑤生理盐水组(n=6),用生理盐水免疫动物,其他处理同完全福氏佐剂组。观察各组大鼠发病和死亡情况,腹腔给药24h后麻醉状态下处死大鼠,取脑、脊髓,用原位末端标记法法检测大鼠脑脊髓细胞凋亡情况,计算凋亡率。结果:42只大鼠全部进入结果分析。①至取材时发病和死亡情况:模型组、地塞米松组和雷公藤甲素组发病率无差异[80%(8/10),90%(9/10),90%(9/10)],但地塞米松组和雷公藤甲素组死亡率低于模型组[0,10%(1/10),40%(4/10)]。②脑组织凋亡细胞率:生理盐水组无凋亡,模型组为(0.97±2.38)%,地塞米松组和雷公藤甲素组均高于完全福氏佐剂组[(47.72±6.38)%,(47.63±8.81)%,(15.98±3.77)%,P<0.05]。③脊髓中细胞凋亡率:生理盐水组和模型组无凋亡;地塞米松组和雷公藤甲素组均高于完全福氏佐剂组[(39.41±7.01)%,(38.70±5.08)%,(14.14±2.34)%,P<0.05]。结论:雷公藤甲素对实验性变态反应性脑脊髓炎有治疗作用,可减低动物死亡率,有促进大鼠脑脊髓中细胞凋亡的作用,其治疗效果与地塞米松相似。
AIM： Apoptosis is one of the causes of the recovery of experimental allergic reaction encephalomyelitis and the remission emerged foLlow-up, to observe after the experimental allergic encephalomyelitis induced by Wistar rats, the therapeutic efficacy of triptolide and its effect on apoptosis in marrowbrain in Wistar rats, and compare with glucocorticoid （GG） dexamethasone. METHODS： The experiment was performed in Center Laboratory of Institude of Brain Science of Shanxi Datong University from March to October 2003. The female Wistar rats were selected and divided randomly into 5 groups： ① Dexamethasone group （n=10）： With 0.4 mL antigen homogenate per piece, animals were taken immunity under skin of feet pad of the double hindlimb, meanwhile the 0.05 mL attenuation pertussis rod were treated subcutaneous injection of sorsum of foot （including 5.0×10^10 thallus）. The dexamethasone 10 mg/kg was injected into abdominal cavity after onset of immunity or the 15^th day after immunity. ② Model group （n =10）： the disposal was the same as before, but 0.5 mL saline was injected into abdominal cavity instead. ③ Triptolide group （n=10）： the disposal was the same as before, but 40μg/kg triptolide was injected into abdominal cavity instead.④ Complete Freund＇s adjuvant group （n=6）： The animals were taken immunity with complete Freund＇s adjuvant （0.4 mL） and attenuation pertussis rod （0.05 mL）, method as before, 0.5 mL saline was injected into abdominal cavity 15 days after immunity. ⑤ Saline group （n=6）： The animals were taken immunity with saline, other disposal the same to complete Freund＇s adjuvant group. The episode and death condition in rats in every group were observed, and the rats were killed 24 hours after administration through abdominal cavity under drugged state to gain the brain and spinal cord. The apoptosis condition of marrowbrain in rats was detected with in situ end-labeling method, and apoptosis rate was calculated. RESULTS： Totally 42 rats were involved in the result analysis.① The episode and death condition till drawing the materials： The incidence rate in model group, dexamethasone group and triptolide group had no difference [80%（8/10）, 90%（9/10）, 90%（9/10）], but the death rate in dexamethasone group and triptolide group was lower than that in model group [0, 10% （1/10）, 40% （4/10）]. ② Apoptosis rate of brain tissue： There was no apoptosis in saline group. In model group it was （0.97±9.38）%, and it was higher in dexamethasone group and triptolide group than that in complete Freund＇s adjuvant group [（47.72±6.38）%, （47.63±8.81）%, （15.98±3.77）%, P 〈 0.05].③ The apoptosis rate in spinal cord： There was no apoptosis in saline group and model group; It was higher in dexamethasone group and triptolide group than that in complete Freund＇s adjuyant group [（39.41±7.01 ）%, （38.70±.08）%, （14.14±9.34）%, P 〈 0.05]. CONCLUSION： Triptolide has therapeutic effect on experimental allergic encephalomyelitis, can decrease the death rate of animal, and has the effect of accelerating the apoptosis in marrowbrain in rats, and its therapeutic efficacy was similar to dexamethasone.
出处
《中国临床康复》
CSCD
北大核心
2005年第29期137-139,共3页
Chinese Journal of Clinical Rehabilitation
基金
2003年山西省归国留学人员科研资金赞助(2003-69)~~
