摘要
目的研究黄芪总皂苷的大孔吸附树脂分离纯化工艺,为黄芪总皂苷的工业化生产提供参考。方法采用AB-8大孔吸附树脂对黄芪总皂苷进行吸附纯化,以黄芪总皂苷的收率、质量分数为考察指标综合评价。结果60%乙醇提取树脂吸附后用不同体积分数乙醇洗脱,黄芪总皂苷主要集中在40%～80%乙醇部位。树脂对黄芪总皂苷的动态吸附率为10.78mg/mL树脂;不同浓度NaOH溶液对杂质的洗脱效果不同,以0.1%NaOH溶液洗脱效果较好。结论黄芪提取物上AB-8树脂柱,以水和0.1%NaOH洗脱后以80%乙醇洗脱黄芪总皂苷部位可使黄芪总皂苷的收率和质量分数达到较满意效果。
Objective Study on purification of total astragalosides by macroporous adsorptive resin to provide evidence for their industrialized production. Methods Total astragalosides were purified by AB-8 macroporous adsorptive resin and evaluated by yield and purity. Results Total astragalosides were mainly in 40%-80% ethanol parts eluted by different concentration ethanol. The resin dynamic adsorption rate was 10. 78 mg/mL; different concentration NaOH solution had different effect to elute impurities and 0. 1% NaOH had better effect. Conclusion The total astragalosides are adsorbed by AB-8 resin collumn, eluted with water, 0. 1% NaOH, then 20% ethanol in order, at last both the total astragalosides yield and purity have desirable higher level in 80% ethanol elution.
出处
《中草药》
CAS
CSCD
北大核心
2005年第9期1322-1325,共4页
Chinese Traditional and Herbal Drugs
基金
广东省教育厅资助课题(B01039)
上海市教委E-研究院建设计划项目资助(E03008)
关键词
黄芪
黄芪总皂苷
树脂吸附
Radix Astragali
total astragalosides
resin adsorption
