摘要
目的为探讨细胞色素P450IA1(CYP450IA1)基因多态性与肺癌易感性的关系。方法应用聚合酶链反应(PCR)方法对149例肺癌患者和208例健康对照者进行了CYP450IA1基因多态性分析。结果肺癌患者CYP4501A1基因高诱导基因型(Val/Val型)携带率达16.1%,显著高于对照组的8.7%。按病理分型分析发现,鳞癌Val/Val型携带率最高,达21.5%,显著高于对照组(8.7%)和腺癌组(8.0%),小细胞癌也达到17.6%。结合吸烟状况和CYP450IA1基因型不同分析患肺癌相对危险度,以非吸烟、非Val/Val型的人患肺癌危险性为1.0,则非吸烟、Val/Val型的人患肺癌危险性为1.6(P>0.05);吸烟、Val/Val型的人患肺癌危险性则达到4.8(P<0.001);在吸烟者中,Val/Val型患肺癌危险性是非Val/Val型的2.2倍(P>0.05)。结论肺癌发生与CYP450IA1高诱导基因型密切相关,CYP4501A1高诱导基因型是宿主肺癌易感性的重要遗传标志之一。
Objective To evaluate the role of polymorphism of CYP450IA1 gene in the susceptibilty of lung cancer. Methods The polymorphism of CYP450IA1 gene in 149 lung cancer patients and 208 healthy controls were investigated by PCR teahnique. Results The high inducibility genotype (Val/Val type )of CYP4501A1 in lung cancer patients was 16.1%, which was significantlly higher than that of controls, 8.7%. According to pathological classification of lung cancer, it was indicated that Val/Val type in squamous carcinomas was the highest, 21.54%, which was significantlly higher than that of controls (8.7%) and adenoearcinomas (8.00%), The Vai/Val type in smaLL celt carcinoma reached 17.6%. The relative risks were estimated according to smoking condition and CYP450IA1 genotype. Taking the risk of the category with non-smoking and non-high inducibility genotype (Val/ Val) to be at a baseline of 1.0, the odds ratio was 1.6 (P>0.05) in non-smoking and Val/Val type; 4.8 in smoking and Val/Val type (P<0.001). The relative risk of Val/Val type was 2.2 times as nonVal/Val type for smoking people. Conclusions Development of lung cancer was associated with the high inducibility genotype of CYP4501A1. And Val/Val genotype of CYP450IA1 gene might be an important host hereditary marker for susceptibility to lung cancer.
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
1996年第1期33-36,共4页
Chinese Journal of Tuberculosis and Respiratory Diseases
基金
中日科研协作课题
